When exposed to harsh environmental conditions, such as chemical toxin or low temperature, the development of Drosophila melanogaster (fruit fly) will be affected. Fruit flies have many suitable features for relevant study. They are relatively easy to differentiate the sexes and to obtain virgin males and females for genetic crosses. The anesthesia can be performed with unsophisticated equipment. Flies have a short life cycle and grow well at room temperature at a lower cost. Propionic acid as a disinfectant is added to the growth medium preventing bacteria or fungi infection during culture process. Little is known about the effect of acid on flies’ development. This study aimed to explore the influence of growth dates, dry weight and body length in wild strain in different propionic acid concentrations. The growth days became distinctive when flies reached pupa and adult stages. They grew in 0.8% propionic acid and control treatments better than those in 1.3% and 1.8% propionic acid treatments. It indicated that propionic acid might play a role in affecting flies’ development. The flies’ total number, day weight and body length were measured and analyzed using Analysis of Variance. The results showed that there was no significant difference in the treatments because the probabilities of all the F-test were greater than standard 0.05. This was attributed to large standard deviations in the treatments. In accordance with the averages of the traits, flies developed in vials containing 0.8% to 1.3% propionic acid better than those in vials with 1.8% propionic acid or those in control (no propionic acid). Further study was proposed to learn more about the effect of propionic acid on flies.